The positive effects of dietary flavonoids on health depend on their bioavailability in the human gut, where the flavonoid-modifying enzymes (FMEs) in gut bacteria play a crucial role in flavonoid metabolism. Thus, to comprehensively examine the role of FMEs in this process, we first constructed a database of potential FMEs containing 6,865 proteins. We identified homologs of these FMEs in gut bacterial genomes and reported species that can potentially modify flavonoids but were not previously...

CONCLUSION: This study reveals a novel regulatory axis involving FOXN3 and E2F5 in AML. FOXN3 acts as a tumor suppressor by regulating E2F5 and modulating downstream MAPK signaling pathways.

Antimicrobial resistance has emerged as a critical global health challenge, necessitating the discovery of new antibiotics. Cannabigerol (CBG) and cannabigerolic acid (CBGA) from Cannabis sativa have shown promising activity as antibacterial agents. In this work, a total of 26 CBG and CBGA derivatives (13 of each) featuring varied terpene chain lengths and substitution patterns were synthesized and characterized; of these, 20 are novel analogs. To determine their structure-activity relationships...

Desorption electrospray ionization (DESI) is a widely used ambient mass spectrometry imaging (MSI) technique valued for its minimal sample preparation and ability to preserve native chemical states. However, achieving single-cell resolution with DESI has been challenging due to relatively low efficiencies of molecular ionization and ion transmission at small spatial scales. Here, we present four distinct implementations that enable single-cell DESI imaging of cultured cells through a combination...

Adipose dysfunction contributes to age-related systemic decline primarily through its adverse effects on energy metabolism, insulin sensitivity, circulating adipokines, and inflammation. Time-restricted feeding (TRF) has emerged as a promising approach to correct adipose and metabolic dysfunction. However, most of these studies were carried out in young animals. Whether TRF could exert similar beneficial effects in the adipose tissue during aging remains unknown. To address this, 18-month-old...